Characterization of a GH Family 20 Exo-β-<i>N</i>-acetylhexosaminidase with Antifungal Activity from <i>Streptomyces avermitilis</i>
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Purification and Characterization of β-N-Acetylhexosaminidase from Rice Seeds
N-Acetyl-β-D-hexosaminidase (β-HexNAc’ase) (EC 3.2.1.52) was purified from rice seeds (Oryza sativa L. var. Dongjin) using ammonium sulfate (80%) precipitation, Sephadex G150, CM-Sephadex, and DEAE-Sephadex chromatography, sequentially. The activities were separated into 7 fractions (F1F7) by CM-Sephadex chromatography. Among them, F6 was further purified to homogeneity with a 13.0% yield and 1...
متن کاملPurification and characterization of isoforms of β-N- acetylhexosaminidase from mungbean seedlings
Three isoforms of β-N-acetylhexosaminidase (β-NAHA), named β-NAHAs I, II and III, were isolated from six-day-old etiolated mungbean (Vigna radiata) seedlings. β-NAHA I was purified to apparent homogeneity by a procedure involving Con A-Sepharose chromatography, chromatofocusing, and gel filtration. β-NAHAs II and III were highly purified. β-NAHAs I, II and III had molecular masses of 135,127 an...
متن کاملGaining insight into the inhibition of glycoside hydrolase family 20 exo-β-N-acetylhexosaminidases using a structural approach.
One useful methodology that has been used to give insight into how chemically synthesized inhibitors bind to enzymes and the reasons underlying their potency is crystallographic studies of inhibitor-enzyme complexes. Presented here is the X-ray structural analysis of a representative family 20 exo-β-N-acetylhexosaminidase in complex with various known classes of inhibitor of these types of enzy...
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in the past couple of decades sociocultural theory of sla and its implications in efl contexts have attracted attentions of research circles worldwide and aroused some controversies. firth and wagner (1997) have questioned the principles of the cognitive view which gives importance to mental constructs in favor of sociocultural view which highlights social and contextual constructs. but if soci...
15 صفحه اولCharacterization of a chitinase with antifungal activity from a native Serratia marcescens B4A
Chitinases have the ability of chitin digestion that constitutes a main compound of the cell wall in many of the phytopathogens such as fungi. In the following investigation, a novel chitinase with antifungal activity was characterized from a native Serratia marcescens B4A. Partially purified enzyme had an apparent molecular mass of 54 kDa. It indicated an optimum activity in pH 5 at 45°C. Enzy...
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ژورنال
عنوان ژورنال: Journal of Applied Glycoscience
سال: 2019
ISSN: 1344-7882,1880-7291
DOI: 10.5458/jag.jag.jag-2019_0001